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CREB (phospho Ser133) antibody

Orthogonal
Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975
Anti-CREB (phospho Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53975
Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975
Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975
Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX53975

Cat. No. GTX53975

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Ser133 of human CREB

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

GTX53975 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX53975 CREB (phospho Ser133) antibody.
Antigen retrieval : High pressure antigen retrieval with 10 mM citrate buffer (pH 6)
Dilution : 1:200

Anti-CREB (phospho Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53975

GTX53975 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX53975 CREB (phospho Ser133) antibody.
Red : Primary antibody
Blue : DAPI

Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

GTX53975 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX53975 CREB (phospho Ser133) antibody.
Antigen retrieval : High pressure antigen retrieval with 10 mM citrate buffer (pH 6)
Dilution : 1:200

Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

GTX53975 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX53975 CREB (phospho Ser133) antibody.
Antigen retrieval : High pressure antigen retrieval with 10 mM citrate buffer (pH 6)
Dilution : 1:200

Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX53975

GTX53975 WB Image

Orthogonal

WB analysis of HeLa cell treated by Anisomycin (25 ug/mL) at 37ºC for 30 minutes after serum-starvation overnight lysates using GTX53975 CREB (phospho Ser133) antibody.
Loading : 25 μg per lane
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399