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CREB (phospho Ser133) antibody

Anti-CREB (phospho Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53975
Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX53975
Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975
Anti-CREB (phospho Ser133) antibody used in Immunoprecipitation (IP). GTX53975
Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

Cat No. GTX53975

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Ser133 of human CREB

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB (phospho Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53975

GTX53975 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX53975 CREB (phospho Ser133) antibody.
Blue : DAPI

Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX53975

GTX53975 WB Image

WB analysis of HeLa cells treated by Anisomycin (25 ug/mL) at 37ºC for 30 minutes after serum-starvation overnight using GTX53975 CREB (phospho Ser133) antibody.
Dilution : 1:2000
Loading : 25μg per lane

Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

GTX53975 IHC-P Image

IHC-P analysis of human appendix tissue using GTX53975 CREB (phospho Ser133) antibody.
Dilution : 1:100

Anti-CREB (phospho Ser133) antibody used in Immunoprecipitation (IP). GTX53975

GTX53975 IP Image

IP analysis of 293T cell lysate using GTX53975 CREB (phospho Ser133) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CREB (phospho Ser133) antibody used in IHC (Paraffin sections) (IHC-P). GTX53975

GTX53975 IHC-P Image

IHC-P analysis of human esophageal tissue using GTX53975 CREB (phospho Ser133) antibody.
Dilution : 1:100

REFERENCE

There are currently no references for CREB (phospho Ser133) antibody (GTX53975). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CREB (phospho Ser133) antibody (GTX53975). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf