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CREB (phospho Ser142) antibody

Anti-CREB (phospho Ser142) antibody used in IHC (Paraffin sections) (IHC-P). GTX55056
Anti-CREB (phospho Ser142) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55056
Anti-CREB (phospho Ser142) antibody used in Western Blot (WB). GTX55056

Cat. No. GTX55056

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of CREB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB (phospho Ser142) antibody used in IHC (Paraffin sections) (IHC-P). GTX55056

GTX55056 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55056 CREB (phospho Ser142) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-CREB (phospho Ser142) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55056

GTX55056 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55056 CREB (phospho Ser142) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-CREB (phospho Ser142) antibody used in Western Blot (WB). GTX55056

GTX55056 WB Image

WB analysis of UV-treated HeLa (A), rat muscle (B) whole cell lysates using GTX55056 CREB (phospho Ser142) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399