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CREST antibody [N1C2]

Anti-CREST antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115785
Anti-CREST antibody [N1C2] used in Immunoprecipitation (IP). GTX115785
Anti-CREST antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115785
Anti-CREST antibody [N1C2] used in Western Blot (WB). GTX115785

Cat. No. GTX115785

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CREST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CREST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620713

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SS18L1 subunit of BAF chromatin remodeling complex , CREST , LP2261

Background

Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CREST antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115785

GTX115785 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST(GTX115785) antibody at 1:500 dilution.

Anti-CREST antibody [N1C2] used in Immunoprecipitation (IP). GTX115785

GTX115785 IP Image

Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 μg of CREST antibody [N1C2] (GTX115785).
Western blot analysis was performed using CREST antibody [N1C2] (GTX115785).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CREST antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115785

GTX115785 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST(GTX115785) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREST antibody [N1C2] used in Western Blot (WB). GTX115785

GTX115785 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [N1C2] (GTX115785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169