GeneTex
United States (US)

CREST antibody [N1C2]

GTX115785_40296_WB_M_brain_18121410_998.jpg
GTX115785_WB_18121410_617.jpg
GTX115785_40296_20151204_IP_18121410_785.jpg
GTX115785_40296_IHC_18121410_314.jpg
GTX115785_40296_IFA_18121410_424.jpg

Cat No. GTX115785

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CREST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ss18L1, Nbaf Chromatin Remodeling Complex Subunit,Crest,Lp2261,Ss18L1

Background

Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX115785_40296_WB_M_brain_18121410_998.jpg

GTX115785 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115785 diluted at 1:1000

GTX115785_WB_18121410_617.jpg

GTX115785 WB Image

Sample (20 ug )
A: Hela whole cell lysate
B: HeLa nucleus 10% SDS PAGE
GTX115785 diluted at 1:1000

GTX115785_40296_20151204_IP_18121410_785.jpg

GTX115785 IP Image

Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 μg of CREST antibody [N1C2] (GTX115785).
Western blot analysis was performed using CREST antibody [N1C2] (GTX115785).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115785_40296_IHC_18121410_314.jpg

GTX115785 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST(GTX115785) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115785_40296_IFA_18121410_424.jpg

GTX115785 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST(GTX115785) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169