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CRF antibody

Anti-CRF antibody used in IHC (Paraffin sections) (IHC-P). GTX31922
Anti-CRF antibody used in IHC (Paraffin sections) (IHC-P). GTX31922
Anti-CRF antibody used in Western Blot (WB). GTX31922

Cat. No. GTX31922

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all CRF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Placenta Tissue Lysate(GTX29186)

Product Note

CRH antibody is human specific.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CRF antibody was raised against a 16 amino acid peptide near the amino terminus of human CRF.The immunogen is located within amino acids 30 - 80 of CRF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

corticotropin releasing hormone , CRF , CRH1

Cellular Localization

Secreted

Background

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-CRF antibody used in IHC (Paraffin sections) (IHC-P). GTX31922

GTX31922 IHC-P Image

IHC-P analysis of human brain tissue using GTX31922 CRF antibody.
Working concentration : 20 μg/ml

Anti-CRF antibody used in IHC (Paraffin sections) (IHC-P). GTX31922

GTX31922 IHC-P Image

IHC-P analysis of human brain tissue using GTX31922 CRF antibody.
Working concentration : 5 μg/ml

Anti-CRF antibody used in Western Blot (WB). GTX31922

GTX31922 WB Image

WB analysis of human placenta tissue lysate using GTX31922 CRF antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399