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CRHR1 (aa 107 - 117) antibody, Internal

Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Western Blot (WB). GTX88961
Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Immunohistochemistry (IHC). GTX88961

Cat. No. GTX88961

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CRHR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
ICC/IF Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88961-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10731029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

corticotropin releasing hormone receptor 1 , CRF-R , CRF-R-1 , CRF-R1 , CRF1 , CRFR-1 , CRFR1 , CRH-R-1 , CRH-R1 , CRHR , CRHR1L

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endosome

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Western Blot (WB). GTX88961

GTX88961 WB Image

WB analysis of human colon (A), cerebellum (B), rat brain (C) and mouse spinal cord (D) lysates using GTX88961 CRHR1 (aa 107 - 117) antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CRHR1 (aa 107 - 117) antibody, Internal used in Immunohistochemistry (IHC). GTX88961

GTX88961 IHC Image

IHC analysis of dendrite of a neuronal cell in C57 mouse cortex using GTX88961 CRHR1 (aa 107 - 117) antibody, Internal.
Green : Primary antibody
Blue : DAPI
Dilution : 0.3μg/ml

REFERENCE

Application Reference

Hiramoto K et al. Photochem Photobiol 2016; 92 (5) : 728-34 The Effects of Ultraviolet Eye Irradiation on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice.
Application : WB
Reactivity : Mouse
Kimura A et al. J Dermatol 2011; 38 (8) : 740-7 Influence of trichloroacetic acid peeling on the skin stress response system.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399