GeneTex
United States (US)

CRHR1 antibody [N1N2], N-term

GTX100180_39391_IHC_19011506_823.jpg
GTX100180_39391_WB_M_Brain_19011506_526.jpg

Cat No. GTX100180

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CRHR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CRF-R antibody, CRF-R-1 antibody, CRF-R1 antibody, CRF1 antibody, CRFR-1 antibody, CRFR1 antibody, CRH-R-1 antibody, CRH-R1 antibody, CRH-R1h antibody, CRHR antibody, CRHR1L antibody, CRHR1f antibody, CRHR1 antibody, seven transmembrane helix receptor antibody, corticotropin releasing hormone receptor variant 1e antibody, corticotropin-releasing factor receptor 1 antibody, corticotropin-releasing factor type 1 receptor antibody, corticotropin-releasing hormone receptor 1 antibody, corticotropin releasing hormone receptor 1 antibody

Cellular Localization

Cell membrane

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES
GTX100180_39391_IHC_19011506_823.jpg

GTX100180 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using CRFR1(GTX100180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100180_39391_WB_M_Brain_19011506_526.jpg

GTX100180 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX100180 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf