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CRHR1 antibody

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

Cat No. GTX130244

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

U87-MG (unboiled) , U87-MG MF (unboiled) , Mouse brain , Rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CRHR1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886219

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

corticotropin releasing hormone receptor 1 , CRF-R , CRF-R-1 , CRF-R1 , CRF1 , CRFR-1 , CRFR1 , CRH-R-1 , CRH-R1 , CRHR , CRHR1L

Cellular Localization

Cell membrane

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

CRHR1 antibody detects CRHR1 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000.

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

CRHR1 antibody detects CRHR1 protein by western blot analysis. U87-MG whole cell extracts and membrane extracts (unboiled) (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000.

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

CRHR1 antibody detects CRHR1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000.

REFERENCE

REVIEW

CRHR1 antibody Cat No. GTX130244

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Transl Psychiatry in 2018.PMID: 29780165
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf