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CRK antibody [3H7-E5-H8]

Anti-CRK antibody [3H7-E5-H8] used in Western Blot (WB). GTX49212

Cat No. GTX49212

Host

Mouse

Clonality

Monoclonal

Clone Name

3H7-E5-H8

Isotype

IgG2b

Application

WB

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human CRK protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Crk Proto-Oncogene, Adaptor Protein , Crkii , P38 , Crk

Cellular Localization

Cytoplasm, Cell membrane

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Database

Research Area

DATA IMAGES

Anti-CRK antibody [3H7-E5-H8] used in Western Blot (WB). GTX49212

GTX49212 WB Image

WB analysis of LNCAP, A431, MCF7 and HT1080 lysates using CRK antibody at a dilution of 1:1,000.

REFERENCE

There are currently no references for CRK antibody [3H7-E5-H8] (GTX49212). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf