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CRK antibody [N2C3]

Comparison
Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901
Anti-CRK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100901
Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901

Cat. No. GTX100901

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CRK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NIH-3T3 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240661

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK proto-oncogene, adaptor protein , CRKII , p38

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901

GTX100901 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 12% SDS PAGE
GTX100901 diluted at 1:1000

Anti-CRK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100901

GTX100901 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CRK(GTX100901) antibody at 1:200 dilution.

Anti-CRK antibody [N2C3] used in Western Blot (WB). GTX100901

GTX100901 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] (GTX100901) diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169