GeneTex
United States (US)

CRK antibody [N2C3]

Comparison
GTX100901_39596_IFA_19011506_433.jpg
GTX100901_WB_M_19011506_697.jpg
GTX100901_39596_20161124_WB_competitor_watermark_19011506_687.jpg

Cat No. GTX100901

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Crk Proto-Oncogene, Adaptor Protein,Crkii,P38,Crk

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100901_39596_IFA_19011506_433.jpg

GTX100901 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CRK(GTX100901) antibody at 1:200 dilution.

GTX100901_WB_M_19011506_697.jpg

GTX100901 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 12% SDS PAGE
GTX100901 diluted at 1:1000

GTX100901_39596_20161124_WB_competitor_watermark_19011506_687.jpg

GTX100901 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] (GTX100901) diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf