*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2 , NIH-3T3
Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Crk Proto-Oncogene, Adaptor Protein,Crkii,P38,Crk
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]
GTX100901 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using CRK(GTX100901) antibody at 1:200 dilution.
GTX100901 WB Image
Sample (30 ug of whole cell lysate)
A:NIH-3T3 12% SDS PAGE
GTX100901 diluted at 1:1000
GTX100901 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] (GTX100901) diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.