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CRMP1 antibody

Orthogonal
Anti-CRMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114940
Anti-CRMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114940
Anti-CRMP1 antibody used in Western Blot (WB). GTX114940
Anti-CRMP1 antibody used in Western Blot (WB). GTX114940

Cat. No. GTX114940

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CRMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Mouse brain , MCF-7

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CRMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collapsin response mediator protein 1 , CRMP-1 , DPYSL1 , DRP-1 , DRP1 , ULIP-3

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CRMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114940

GTX114940 IHC-P Image

CRMP1 antibody detects CRMP1 protein at cytosol on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
CRMP1 antibody (GTX114940) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CRMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114940

GTX114940 IHC-P Image

CRMP1 antibody detects CRMP1 protein at on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CRMP1 antibody (GTX114940) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CRMP1 antibody used in Western Blot (WB). GTX114940

GTX114940 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX114940 diluted at 1:10000

Anti-CRMP1 antibody used in Western Blot (WB). GTX114940

GTX114940 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX114940 diluted at 1:1000

REFERENCE

Application Reference

Lin YF et al. Int J Mol Sci 2023; 24 (5) Elevated Hippocampal CRMP5 Mediates Chronic Stress-Induced Cognitive Deficits by Disrupting Synaptic Plasticity, Hindering AMPAR Trafficking, and Triggering Cytokine Release.
Application : WB
Reactivity : Mouse
Lin YS et al. Neuropharmacology 2019; 157 : 107673 Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease.
Application : WB
Submit a Reference

REVIEW

CRMP1 antibody Cat. No. GTX114940

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-CRMP1 antibody used in Western Blot (WB). GTX114940
Date : Anonymous submitted on 30-Aug-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : hippocampal neuron cells
Amount used : 15μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169