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CRMP2 antibody [N1N3]

Anti-CRMP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113420
Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420
Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420
Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420
Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

Cat No. GTX113420

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CRMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036670

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dihydropyrimidinase like 2 , CRMP-2 , CRMP2 , DHPRP2 , DRP-2 , DRP2 , N2A3 , ULIP-2 , ULIP2

Cellular Localization

Cytoplasm

Background

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Database

Research Area

DATA IMAGES

Anti-CRMP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113420

GTX113420 ICC/IF Image

CRMP2 antibody [N1N3] detects CRMP2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

GTX113420 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using CRMP2(GTX113420) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420

GTX113420 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420

GTX113420 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

GTX113420 IHC-P Image

CRMP2 antibody [N1N3] detects CRMP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169