APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 - 1:2000 |
| 1:250 |
| Assay dependent |
| 1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Not reactive with other CRMP isoforms.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
CRMP5 protein purified from human brain.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dihydropyrimidinase like 5 , CRAM , CRMP-5 , CRMP5 , Ulip6
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
Database
Research Area
DATA IMAGES
![Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378](/templates/default/js/lightbox/images/loading.gif "Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378")
|
GTX19378 IHC-P Image
IHC-P analysis of human glioma tissue using GTX19378 CRMP5 antibody [CR-1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50
|
|
|
GTX19378 ICC/IF Image
ICC/IF analysis of SH-SY5Y cells using GTX19378 CRMP5 antibody [CR-1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
|
|
|
GTX19378 IHC-P Image
IHC-P analysis of human brain tissue using GTX19378 CRMP5 antibody [CR-1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
|
|
|
GTX19378 WB Image
WB analysis of tissue whole cell extracts (30 μg lysate) of Mouse Brain (Lane 1), Rat Brain (lane 2), SH-SY5Y (lane 3), NCCIT (Lane 4), NTERA 2 (Lane 5), and PC 12 (lane 6) using GTX19378 CRMP5 antibody [CR-1].
Dilution : 1:1000
|
REFERENCE
There are currently no references for CRMP5 antibody [CR-1] (GTX19378). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CRMP5 antibody [CR-1] (GTX19378). Be the first to share your experience with this product.
![Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378](/upload/website/prouct_img/small/GTX19378/GTX19378_1073_IHC-P_w_23060620_967.webp "Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19378](/upload/website/prouct_img/small/GTX19378/GTX19378_369_ICC-IF_w_23060620_629.webp "Anti-CRMP5 antibody [CR-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378](/upload/website/prouct_img/small/GTX19378/GTX19378_1072_IHC-P_w_23060620_700.webp "Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in Western Blot (WB). GTX19378](/upload/website/prouct_img/small/GTX19378/GTX19378_1571_WB_w_23060620_857.webp "Anti-CRMP5 antibody [CR-1] used in Western Blot (WB). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378](/upload/website/prouct_img/normal/GTX19378/GTX19378_1073_IHC-P_w_23060620_967.webp "Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19378](/upload/website/prouct_img/normal/GTX19378/GTX19378_369_ICC-IF_w_23060620_629.webp "Anti-CRMP5 antibody [CR-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378](/upload/website/prouct_img/normal/GTX19378/GTX19378_1072_IHC-P_w_23060620_700.webp "Anti-CRMP5 antibody [CR-1] used in IHC (Paraffin sections) (IHC-P). GTX19378")
![Anti-CRMP5 antibody [CR-1] used in Western Blot (WB). GTX19378](/upload/website/prouct_img/normal/GTX19378/GTX19378_1571_WB_w_23060620_857.webp "Anti-CRMP5 antibody [CR-1] used in Western Blot (WB). GTX19378")