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CRMP5 antibody [N1C1]

Anti-CRMP5 antibody [N1C1] used in Western Blot (WB). GTX112287
Anti-CRMP5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112287
Anti-CRMP5 antibody [N1C1] used in Western Blot (WB). GTX112287
Anti-CRMP5 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112287
Anti-CRMP5 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112287

Cat. No. GTX112287

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CRMP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRMP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950143

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydropyrimidinase like 5 , CRAM , CRMP-5 , CRMP5 , Ulip6

Cellular Localization

Cytoplasm

Background

Members of the CRMP family, such as DPYSL5, are believed to play a role in growth cone guidance during neural development.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CRMP5 antibody [N1C1] used in Western Blot (WB). GTX112287

GTX112287 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP5 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112287

GTX112287 ICC/IF Image

CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CRMP5 antibody [N1C1] used in Western Blot (WB). GTX112287

GTX112287 WB Image

Mouse tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP5 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112287

GTX112287 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CRMP5(GTX112287) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CRMP5 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX112287

GTX112287 IHC-P Image

CRMP5 antibody [N1C1] detects CRMP5 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zeng HY et al. Graefes Arch Clin Exp Ophthalmol 2019; (Epub) Detection of serum anti-retinal antibodies in the Chinese patients with presumed autoimmune retinopathy.
Application : WB
Reactivity : Human
Saito M et al. BMC Ophthalmol 2014; 14 : 5 A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169