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CROT antibody [N2C2], Internal

Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243
Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243
Anti-CROT antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105243

Cat. No. GTX105243

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CROT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carnitine O-octanoyltransferase , COT

Cellular Localization

Peroxisome

Background

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Database

Research Area

DATA IMAGES

Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243

GTX105243 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
COT antibody
GTX105243 diluted at 1:1000

Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243

GTX105243 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX105243 diluted at 1:1000

Anti-CROT antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105243

GTX105243 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT(GTX105243) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169