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CROT antibody [N2C2], Internal

Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243
Anti-CROT antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105243
Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243

Cat No. GTX105243

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji , mouse liver

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

carnitine O-octanoyltransferase , COT

Cellular Localization

Peroxisome

Background

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Database

Research Area

DATA IMAGES
Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243

GTX105243 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
COT antibody
GTX105243 diluted at 1:1000

Anti-CROT antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105243

GTX105243 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT(GTX105243) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CROT antibody [N2C2], Internal used in Western Blot (WB). GTX105243

GTX105243 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX105243 diluted at 1:1000

REFERENCE
There are currently no references for CROT antibody [N2C2], Internal (GTX105243). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CROT antibody [N2C2], Internal (GTX105243). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169