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CRY2 antibody, N-term

Anti-CRY2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45786
Anti-CRY2 antibody, N-term used in Western Blot (WB). GTX45786

Cat No. GTX45786

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

U937

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human CRY2

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cryptochrome circadian regulator 2 , HCRY2 , PHLL2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-CRY2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45786

GTX45786 IHC-P Image

IHC-P analysis of human heart tissue using GTX45786 CRY2 antibody at 1:100. Left to right:DAPI, CRY2 , Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

Anti-CRY2 antibody, N-term used in Western Blot (WB). GTX45786

GTX45786 WB Image

WB analysis of U937 cells using GTX45786 CRY2 antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf