APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.2M Tris, 0.2M Glycine, 1mM EGTA, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CSB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ERCC excision repair 6, chromatin remodeling factor , ARMD5 , CKN2 , COFS , COFS1 , CSB , CSB-PGBD3 , POF11 , RAD26 , UVSS1
Cellular Localization
Nucleus
Background
This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity; there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589](/upload/website/prouct_img/middle/GTX104589/GTX104589_41649_20150612_IP_19011506_278.jpg "Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589")
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GTX104589 IP Image
Immunoprecipitation of CSB protein from 293T whole cell extracts using 5 μg of CSB antibody [N2C1], Internal (GTX104589).
Western blot analysis was performed using CSB antibody [N2C1], Internal (GTX104589).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
![Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589](/upload/website/prouct_img/middle/GTX104589/GTX104589_41649_20150525_IFA_19011506_940.jpg "Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589")
|
GTX104589 ICC/IF Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
|
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/middle/GTX104589/GTX104589_41038_IHC_R_19011506_336.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
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GTX104589 IHC-P Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/middle/GTX104589/GTX104589_41038_IHC_M_19011506_538.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
|
GTX104589 IHC-P Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
![Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589](/upload/website/prouct_img/middle/GTX104589/GTX104589_41697_20190809_WB_19120122_982.jpg "Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589")
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GTX104589 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CSB antibody [N2C1], Internal (GTX104589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CSB antibody [N2C1], Internal (GTX104589). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CSB antibody [N2C1], Internal (GTX104589). Be the first to share your experience with this product.
![Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589](/upload/website/prouct_img/small/GTX104589/GTX104589_41649_20150612_IP_19011506_278.jpg "Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589](/upload/website/prouct_img/small/GTX104589/GTX104589_41649_20150525_IFA_19011506_940.jpg "Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/small/GTX104589/GTX104589_41038_IHC_R_19011506_336.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/small/GTX104589/GTX104589_41038_IHC_M_19011506_538.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589](/upload/website/prouct_img/small/GTX104589/GTX104589_41697_20190809_WB_19120122_982.jpg "Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589](/upload/website/prouct_img/normal/GTX104589/GTX104589_41649_20150612_IP_19011506_278.jpg "Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589](/upload/website/prouct_img/normal/GTX104589/GTX104589_41649_20150525_IFA_19011506_940.jpg "Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/normal/GTX104589/GTX104589_41038_IHC_R_19011506_336.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589](/upload/website/prouct_img/normal/GTX104589/GTX104589_41038_IHC_M_19011506_538.jpg "Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589")
![Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589](/upload/website/prouct_img/normal/GTX104589/GTX104589_41697_20190809_WB_19120122_982.jpg "Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589")
