APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 0.2M Tris, 0.2M Glycine, 1mM EGTA, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CSB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ARMD5 antibody, CKN2 antibody, COFS antibody, COFS1 antibody, CSB antibody, RAD26 antibody, ERCC6 antibody, Cockayne syndrome B protein antibody, ATP-dependent helicase ERCC6 antibody, cockayne syndrome protein CSB antibody, Rad26 homolog antibody, Cockayne syndrome group B protein antibody, DNA excision repair protein ERCC-6 antibody, "excision repair cross-complementing rodent repair deficiency, complementation group 6 antibody"
Cellular Localization
Nucleus
Background
This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity; there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104589 IHC-P Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104589 IHC-P Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104589 IP Image
Immunoprecipitation of CSB protein from 293T whole cell extracts using 5 μg of CSB antibody [N2C1], Internal (GTX104589).
Western blot analysis was performed using CSB antibody [N2C1], Internal (GTX104589).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX104589 WB Image
CSB antibody [N2C1], Internal detects CSB protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500
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GTX104589 ICC/IF Image
CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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