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CSB antibody [N2C1], Internal

Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589
Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589
Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589
Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589
Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589
Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589
Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589

Cat. No. GTX104589

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 6, chromatin remodeling factor , ARMD5 , CKN2 , COFS , COFS1 , CSB , CSB-PGBD3 , POF11 , RAD26 , UVSS1

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity; there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CSB stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CSB antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX104589

GTX104589 IP Image

Immunoprecipitation of CSB protein from 293T whole cell extracts using 5 μg of CSB antibody [N2C1], Internal (GTX104589).
Western blot analysis was performed using CSB antibody [N2C1], Internal (GTX104589).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CSB stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CSB antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104589

GTX104589 ICC/IF Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CSB antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104589

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CSB antibody [N2C1], Internal used in Western Blot (WB). GTX104589

GTX104589 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CSB antibody [N2C1], Internal (GTX104589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Szepanowski LP et al. Cells 2024; 13 (7) Cockayne Syndrome Patient iPSC-Derived Brain Organoids and Neurospheres Show Early Transcriptional Dysregulation of Biological Processes Associated with Brain Development and Metabolism.
Wie M et al. NAR Cancer 2023; 5 (3) : zcad042 Alkylation of nucleobases by 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) sensitizes PARP1-deficient tumors.
Lin H et al. Nat Commun 2021; 12 (1) : 2461 IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.
Reactivity : Mouse
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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169