GeneTex
United States (US)

CSB antibody [N2C1], Internal

GTX104589_41038_IHC_R_19011506_336.jpg
GTX104589_41038_IHC_M_19011506_538.jpg
GTX104589_41649_20150612_IP_19011506_278.jpg
GTX104589_41650_WB_19011506_589.jpg
GTX104589_41649_20150525_IFA_19011506_940.jpg

Cat No. GTX104589

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 0.2M Tris, 0.2M Glycine, 1mM EGTA, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARMD5 antibody, CKN2 antibody, COFS antibody, COFS1 antibody, CSB antibody, RAD26 antibody, ERCC6 antibody, Cockayne syndrome B protein antibody, ATP-dependent helicase ERCC6 antibody, cockayne syndrome protein CSB antibody, Rad26 homolog antibody, Cockayne syndrome group B protein antibody, DNA excision repair protein ERCC-6 antibody, "excision repair cross-complementing rodent repair deficiency, complementation group 6 antibody"

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The protein has ATP-stimulated ATPase activity; there are contradictory publications reporting presence or absence of helicase activity. The protein appears to interact with several transcription and excision repair proteins, and may promote complex formation at repair sites. Mutations in this gene result in Cockayne syndrome type B. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104589_41038_IHC_R_19011506_336.jpg

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104589_41038_IHC_M_19011506_538.jpg

GTX104589 IHC-P Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104589_41649_20150612_IP_19011506_278.jpg

GTX104589 IP Image

Immunoprecipitation of CSB protein from 293T whole cell extracts using 5 μg of CSB antibody [N2C1], Internal (GTX104589).
Western blot analysis was performed using CSB antibody [N2C1], Internal (GTX104589).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104589_41650_WB_19011506_589.jpg

GTX104589 WB Image

CSB antibody [N2C1], Internal detects CSB protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500

GTX104589_41649_20150525_IFA_19011506_940.jpg

GTX104589 ICC/IF Image

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169