Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CSB antibody [N2C1], Internal (GTX104589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CSB antibody [N2C1], Internal detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSB protein stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CSB stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of CSB protein from 293T whole cell extracts using 5 μg of CSB antibody [N2C1], Internal (GTX104589).
Western blot analysis was performed using CSB antibody [N2C1], Internal (GTX104589).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

CSB antibody [N2C1], Internal detects CSB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CSB stained by CSB antibody [N2C1], Internal (GTX104589) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CSB antibody [N2C1], Internal detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CSB antibody [N2C1], Internal (GTX104589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min