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CSDA antibody

Anti-CSDA antibody used in Western Blot (WB). GTX130052
Anti-CSDA antibody used in IHC (Paraffin sections) (IHC-P). GTX130052
Anti-CSDA antibody used in IHC (Paraffin sections) (IHC-P). GTX130052
Anti-CSDA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130052
Anti-CSDA antibody used in Western Blot (WB). GTX130052

Cat. No. GTX130052

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CSDA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Observed MW

40 | 48 kDa. The observed M.W. is based on the following publication. PMID: 26646932

Positive Control

mouse muscle , rat heart

Predict Reactivity

Bovine, Dog, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CSDA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886164

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Y-box binding protein 3 , CSDA , CSDA1 , DBPA , ZONAB

Cellular Localization

Cytoplasm , Nucleus

Background

Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression.

Database

Research Area

DATA IMAGES

Anti-CSDA antibody used in Western Blot (WB). GTX130052

GTX130052 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CSDA antibody (GTX130052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSDA antibody used in IHC (Paraffin sections) (IHC-P). GTX130052

GTX130052 IHC-P Image

CSDA antibody detects CSDA protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat salivary gland.
CSDA stained by CSDA antibody (GTX130052) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CSDA antibody used in IHC (Paraffin sections) (IHC-P). GTX130052

GTX130052 IHC-P Image

CSDA antibody detects CSDA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CSDA stained by CSDA antibody (GTX130052) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CSDA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130052

GTX130052 ICC/IF Image

CSDA antibody detects CSDA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSDA stained by CSDA antibody (GTX130052) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CSDA antibody used in Western Blot (WB). GTX130052

GTX130052 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with YBX3 antibody (GTX130052) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16603261

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169