APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CSE1L. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888206
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chromosome segregation 1 like , CAS , CSE1 , XPO2
Cellular Localization
Cytoplasm , Nucleus
Background
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/templates/default/js/lightbox/images/loading.gif "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
|
GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extrac
E. 30 μg BCL-1 whole cell lysate/extrac
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
|
|
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GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
|
|
|
GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
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|
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GTX630395 ICC/IF Image
CSE1L antibody [GT729] detects CSE1L protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CSE1L stained by CSE1L antibody [GT729] (GTX630395) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10μm.
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REFERENCE
There are currently no references for CSE1L antibody [GT729] (GTX630395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CSE1L antibody [GT729] (GTX630395). Be the first to share your experience with this product.
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_M_w_23061202_500.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_R_w_23061202_218.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_w_23061202_271.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_20180321_ICC_IF_w_23061202_861.webp "Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_M_w_23061202_500.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_R_w_23061202_218.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_w_23061202_271.webp "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_20180321_ICC_IF_w_23061202_861.webp "Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395")