APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CSE1L. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888206
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
chromosome segregation 1 like , CAS , CSE1 , XPO2
Cellular Localization
Cytoplasm , Nucleus
Background
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395](/templates/default/js/lightbox/images/loading.gif "Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395")
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GTX630395 ICC/IF Image
CSE1L antibody [GT729] detects CSE1L protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CSE1L stained by CSE1L antibody [GT729] (GTX630395) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10μm.
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GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
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GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extrac
E. 30 μg BCL-1 whole cell lysate/extrac
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
|
|
|
GTX630395 WB Image
CSE1L antibody [GT729] detects CSE1L protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
CSE1L antibody [GT729] (GTX630395) dilution: 1:1000
|
REFERENCE
There are currently no references for CSE1L antibody [GT729] (GTX630395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CSE1L antibody [GT729] (GTX630395). Be the first to share your experience with this product.
![Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_20180321_ICC_IF_18121411_575.jpg "Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_R_18121411_816.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_M_18121411_160.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/small/GTX630395/GTX630395_41526_WB_18121411_330.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_20180321_ICC_IF_18121411_575.jpg "Anti-CSE1L antibody [GT729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_R_18121411_816.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_M_18121411_160.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")
![Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395](/upload/website/prouct_img/normal/GTX630395/GTX630395_41526_WB_18121411_330.jpg "Anti-CSE1L antibody [GT729] used in Western Blot (WB). GTX630395")