GeneTex

  • Country / Location Selection

United States (US)

CSE1L antibody

KO/KD
Anti-CSE1L antibody used in Western Blot (WB). GTX103005
Anti-CSE1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103005
Anti-CSE1L antibody used in Western Blot (WB). GTX103005
Anti-CSE1L antibody used in Western Blot (WB). GTX103005
Anti-CSE1L antibody used in IHC (Paraffin sections) (IHC-P). GTX103005
Anti-CSE1L antibody used in Western Blot (WB). GTX103005

Cat. No. GTX103005

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CSE1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , C2C12 , PC-12 , Rat2 , 293T

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSE1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950046

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromosome segregation 1 like , CAS , CSE1 , XPO2

Cellular Localization

Cytoplasm , Nucleus

Background

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CSE1L antibody used in Western Blot (WB). GTX103005

GTX103005 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CSE1L antibody (GTX103005) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSE1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103005

GTX103005 ICC/IF Image

CSE1L antibody detects CSE1L protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CSE1L stained by CSE1L antibody (GTX103005) diluted at 1:1500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-CSE1L antibody used in Western Blot (WB). GTX103005

GTX103005 WB Image

CSE1L antibody detects CSE1L protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
CSE1L antibody (GTX103005) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSE1L antibody used in Western Blot (WB). GTX103005

GTX103005 WB Image

CSE1L antibody detects CSE1L protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
CSE1L antibody (GTX103005) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSE1L antibody used in IHC (Paraffin sections) (IHC-P). GTX103005

GTX103005 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using CSE1L(GTX103005) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CSE1L antibody used in Western Blot (WB). GTX103005

GTX103005 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CSE1L antibody (GTX103005) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tuncay Tunccan et al. BJORL 2019; (Epub) The role of CSE1L expression in cervical lymph node metastasis of larynx tumorsO papel da express?o de CSE1L na met?stase em linfonodos cervicais detumores lar?ngeo.
Application : IHC-P
Reactivity : Human
Wang L et al. Cell Death Dis 2018; 9 (10) : 1005 A pharmacological probe identifies cystathionine β-synthase as a new negative regulator for ferroptosis.
Application : WB
Reactivity : Human
Tsao HW et al. Proc Natl Acad Sci U S A 2013; 110 (39) : 15776-81 Ets-1 facilitates nuclear entry of NFAT proteins and their recruitment to the IL-2 promoter.
Application : IP
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for CSE1L antibody (GTX103005). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169