GeneTex
United States (US)

CSK antibody [CSK-04]

GTX14996_WB_18121410_232.jpg
CSK-antibody-CSK-04__GTX14996-1_18121409_423.png

Cat No. GTX14996

Host Mouse
Clonality Monoclonal
Clone Name CSK-04
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

IP: Use at an assay dependent dilution. The immunosorbent ( GTX14996 covalently bound to beads) immunoprecipitates CSK from cell lysates. WB: Use at a dilution of 1/250 - 1/300. Predicted molecular weight: 54 kDa. Weak non-specific band of approx. 60 kDa is detected in human cell lines in lower antibody dilutions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment: VSDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL, corresponding to amino acids 330-450 of Human CSK.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-Terminal Src Kinase,Csk

Cellular Localization

Nuclear

Background

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C-terminal tyrosine of Src family kinases thereby down-regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein).

Database

Research Area

DATA IMAGES
GTX14996_WB_18121410_232.jpg

GTX14996 WB Image

Sample: EL-4 mouse cell line.
GTX14996 diluted at 1:250.

CSK-antibody-CSK-04__GTX14996-1_18121409_423.png

GTX14996 ICC/IF Image

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (GTX14996; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Package List Price ($)
$ 319