GeneTex

  • Country / Location Selection

United States (US)

CSK antibody

KO/KD
Anti-CSK antibody used in Western Blot (WB). GTX107916
Anti-CSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107916
Anti-CSK antibody used in Western Blot (WB). GTX107916
Anti-CSK antibody used in IHC (Paraffin sections) (IHC-P). GTX107916

Cat. No. GTX107916

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CSK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , H1299 , Molt-4

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036680

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-terminal Src kinase

Cellular Localization

Cytoplasm , Cell membrane

Background

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

Database

Research Area

DATA IMAGES

Anti-CSK antibody used in Western Blot (WB). GTX107916

GTX107916 WB Image

KO/KD

Wild-type (WT) and CSK knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CSK antibody (GTX107916) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107916

GTX107916 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using c-Src(GTX107916) antibody at 1:200 dilution.

Anti-CSK antibody used in Western Blot (WB). GTX107916

GTX107916 WB Image

Sample (30μg whole cell lysate)
A:293T
B:H1299
C:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107916 diluted at 1:1000

Anti-CSK antibody used in IHC (Paraffin sections) (IHC-P). GTX107916

GTX107916 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using c-Src(GTX107916) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Navare AT et al. J Cell Biol 2022; 221 (11) Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Application : WB
Reactivity : Human
Navare AT et al. bioRxiv 2020; Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for CSK antibody (GTX107916). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169