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CSK antibody

Anti-CSK antibody used in IHC (Paraffin sections) (IHC-P). GTX74502
Anti-CSK antibody used in Western Blot (WB). GTX74502
Anti-CSK antibody used in Western Blot (WB). GTX74502

Cat. No. GTX74502

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all CSK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml
IHC-P 0.1-0.3μg/ml
ELISA >1/32,000

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 9.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the C-terminal region of CSK (c-src tyrosine kinase).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_377676

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-terminal Src kinase

Cellular Localization

Cytoplasm , Cell membrane

Background

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CSK antibody used in IHC (Paraffin sections) (IHC-P). GTX74502

GTX74502 IHC-P Image

IHC-P analysis of human colon tissue using GTX74502 CSK antibody. Antigen retrieval using citrate buffer at pH6.0.

Anti-CSK antibody used in Western Blot (WB). GTX74502

GTX74502 WB Image

WB analysis of mouse spleen tissue lysate using GTX74502 CSK antibody at 1 μg/ml

Anti-CSK antibody used in Western Blot (WB). GTX74502

GTX74502 WB Image

WB analysis of Jurkat cell lysate using GTX74502 CSK antibody at 0.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399