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CSRP3 antibody

Anti-CSRP3 antibody used in Western Blot (WB). GTX110536
Anti-CSRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110536
Anti-CSRP3 antibody used in Western Blot (WB). GTX110536
Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536
Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536
Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536

Cat No. GTX110536

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSRP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950051

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cysteine and glycine rich protein 3 , CLP , CMD1M , CMH12 , CRP3 , LMO4 , MLP

Cellular Localization

Nucleus , Cytoplasm , cytoskeleton

Background

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CSRP3 antibody used in Western Blot (WB). GTX110536

GTX110536 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody (GTX110536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CSRP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110536

GTX110536 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CSRP3(GTX110536) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-CSRP3 antibody used in Western Blot (WB). GTX110536

GTX110536 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX110536 diluted at 1:1000

Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536

GTX110536 IHC-P Image

CSRP3 antibody detects CSRP3 protein at cytosol on C2C12 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12 xenograft.
CSRP3 antibody (GTX110536) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536

GTX110536 IHC-P Image

CSRP3 antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
CSRP3 stained by CSRP3 antibody (GTX110536) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CSRP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110536

GTX110536 IHC-P Image

CSRP3 antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CSRP3 stained by CSRP3 antibody (GTX110536) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169