GeneTex
United States (US)

CSTF2 antibody [N1N2], N-term

GTX114994_40240_IHC_18121410_124.jpg
GTX114994_40240_IFA_18121410_897.jpg
GTX114994_WB_18121410_109.jpg

Cat No. GTX114994

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CSTF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CstF-64 antibody, CSTF2 antibody, cleavage stimulation factor 64 kDa subunit antibody, betaCstF-64 variant 2 antibody, CF-1 64 kDa subunit antibody, cleavage stimulation factor subunit 2 antibody, CSTF 64 kDa subunit antibody, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa antibody

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq]
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Database

DATA IMAGES
GTX114994_40240_IHC_18121410_124.jpg

GTX114994 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using Cleavage stimulation factor 2(GTX114994) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114994_40240_IFA_18121410_897.jpg

GTX114994 ICC/IF Image

CSTF2 antibody [N1N2], N-term detects CSTF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CSTF2 protein stained by CSTF2 antibody [N1N2], N-term (GTX114994) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX114994_WB_18121410_109.jpg

GTX114994 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX114994 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169