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CTCF antibody

Anti-CTCF antibody used in Western Blot (WB). GTX65926
Anti-CTCF antibody used in Immunoprecipitation (IP). GTX65926
Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926
Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926
Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926
Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926
Anti-CTCF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65926
Anti-CTCF antibody used in ChIP assay (ChIP assay). GTX65926
Anti-CTCF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65926

Cat. No. GTX65926

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all CTCF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
ChIP assay 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCCTC-binding factor , MRD21

Cellular Localization

nucleoplasm

Background

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CTCF antibody used in Western Blot (WB). GTX65926

GTX65926 WB Image

WB analysis of 293T cell lysate using GTX65926 CTCF antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CTCF antibody used in Immunoprecipitation (IP). GTX65926

GTX65926 IP Image

IP analysis of HeLa cell lysate using GTX65926 CTCF antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926

GTX65926 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX65926 CTCF antibody.
Antigen retrieval : 0.01M Citrate Buffer, pH 6.0
Dilution : 1:100

Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926

GTX65926 IHC-P Image

IHC-P analysis of mouse colon tissue using GTX65926 CTCF antibody.
Antigen retrieval : 0.01M Citrate Buffer, pH 6.0
Dilution : 1:100

Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926

GTX65926 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX65926 CTCF antibody.
Antigen retrieval : 0.01M Citrate Buffer, pH 6.0
Dilution : 1:100

Anti-CTCF antibody used in IHC (Paraffin sections) (IHC-P). GTX65926

GTX65926 IHC-P Image

IHC-P analysis of human normal cervix tissue using GTX65926 CTCF antibody.
Antigen retrieval : 0.01M Citrate Buffer, pH 6.0
Dilution : 1:100

Anti-CTCF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65926

GTX65926 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX65926 CTCF antibody.
Blue : DAPI
Dilution : 1:100

Anti-CTCF antibody used in ChIP assay (ChIP assay). GTX65926

GTX65926 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX65926 CTCF antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-CTCF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65926

GTX65926 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65926 CTCF antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419