GeneTex

  • Country / Location Selection

United States (US)

CTGF antibody

Orthogonal
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in IHC (Paraffin sections) (IHC-P). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Anti-CTGF antibody used in Western Blot (WB). GTX124232

Cat. No. GTX124232

Reference 23
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Bovine, Baboon
Package
100 μl ($399),
25 μl ($169)
See all CTGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Sheep, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169640

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular communication network factor 2 , CTGF , HCS24 , IGFBP8 , NOV2

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTGF antibody used in IHC (Paraffin sections) (IHC-P). GTX124232

GTX124232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CTGF(GTX124232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Orthogonal

The data was published in the J Cell Mol Med in 2020. PMID: 32827244

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Orthogonal

The data was published in the 2020 in Antioxidants (Basel). PMID: 33203103

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Orthogonal

The data was published in the J Cell Mol Med in 2020. PMID: 32827244

Anti-CTGF antibody used in Western Blot (WB). GTX124232

GTX124232 WB Image

Orthogonal

The data was published in the J Cell Mol Med in 2020. PMID: 32827244

REFERENCE

Application Reference

Perego MC et al. Toxicol Res (Camb) 2023; 12 (2) : 296-309 Arsenic impairs stem cell differentiation via the Hippo signaling pathway.
Schneider M et al. Matrix Biol 2021; 97 : 1-19 A novel ocular function for decorin in the aqueous humor outflow.
Application : IHC-Fr
Reactivity : Mouse
Arellano-Buend?a AS et al. Antioxidants (Basel) 2020; 9 (11) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.
Application : WB
Reactivity : Rat
Huang S et al. J Cell Mol Med 2020; 24 (19) : 11133-11145 Up-regulated acylglycerol kinase (AGK) expression associates with gastric cancer progression through the formation of a novel YAP1-AGK-positive loop.
Application : WB
Reactivity : Human
Miyazaki N et al. Nutrients 2020; 12 (5) Blueberry Prevents the Bladder Dysfunction in Bladder Outlet Obstruction Rats by Attenuating Oxidative Stress and Suppressing Bladder Remodeling.
Application : WB
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

CTGF antibody Cat. No. GTX124232

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Date : Anonymous submitted on 17-Aug-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : liver tissue
Amount used : 50μg
Blocking : Odyssey Blocking Buffer (No Tween), 25°C, 1Hr
Primary Antibody : 1μg/ml, 4°C, 16Hr
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Date : Anonymous submitted on 23-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : small intestine tissue
Amount used : 40μg
Primary Antibody : 1:500, 4°C, 16Hr
Anti-CTGF antibody used in Western Blot (WB). GTX124232
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample : CCD18co cells mouse intestine tissue
Amount used : 20μg
Blocking : 5% non fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
Submit a Review
Application Reference
Perego MC et al. Toxicol Res (Camb) 2023; 12 (2):296-309 Arsenic impairs stem cell differentiation via the Hippo signaling pathway.
Schneider M et al. Matrix Biol 2021; 97:1-19 A novel ocular function for decorin in the aqueous humor outflow.
Application : IHC-Fr
Reactivity : Mouse
Arellano-Buend?a AS et al. Antioxidants (Basel) 2020; 9 (11) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.
Application : WB
Reactivity : Rat
Huang S et al. J Cell Mol Med 2020; 24 (19):11133-11145 Up-regulated acylglycerol kinase (AGK) expression associates with gastric cancer progression through the formation of a novel YAP1-AGK-positive loop.
Application : WB
Reactivity : Human
Miyazaki N et al. Nutrients 2020; 12 (5) Blueberry Prevents the Bladder Dysfunction in Bladder Outlet Obstruction Rats by Attenuating Oxidative Stress and Suppressing Bladder Remodeling.
Application : WB
Reactivity : Rat
Zhu L et al. Gene 2020; 742:144556
Application : WB, IHC-P
Reactivity : Human
Mohankumar K et al. Endocrinology 2020; 161 (4) Bis-Indole-Derived Nuclear Receptor 4A1 (NR4A1, Nur77) Ligands as Inhibitors of Endometriosis.
Application : WB
Reactivity : Human
Noe N et al. Lab Invest 2019; (Epub) Mesenchyme-specific deletion of Tgf-β1 in the embryonic lung disrupts branching morphogenesis and induces lung hypoplasia.
Application : IHC-P
Reactivity : Mouse
Nakai K et al. J Dermatol Sci 2019; 94 (1):205-212 COA-Cl prevented TGF-β1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis.
Application : WB
Reactivity : Human
Canaud G et al. Sci Transl Med 2019; 11 (476) Cyclin G1 and TASCC regulate kidney epithelial cell G2-M arrest and fibrotic maladaptive repair.
Application : WB
Reactivity : Human
Gao L et al. Anim Reprod Sci. 2018; 199:30-39 PTGFR activation promotes the expression of PTGS-2 and growth factors via activation of the PKC signaling pathway in bovine endometrial epithelial cells.
Application : WB
Reactivity : Bovine
Poli G et al. FASEB J 2018;:fj201801028RR Human fetal adrenal cells retain age-related stem- and endocrine-differentiation potential in culture.
Application : IHC-P
Reactivity : Human
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Zhang S et al. Reprod Domest Anim 2017; (Epub) Concentration effect of prostaglandin E2 on the growth factor expression and cell proliferation in bovine endometrial explants and their kinetic characteristics.
Gao L et al. Anim Reprod Sci 2017; (Epub) PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle.
Application : WB
Reactivity : Bovine
Lee WY et al. Int J Cancer 2017; 141 (9):1921-1931 * sensitizes kras-mutant non-small-cell lung cancer to * by targeting taz.
Application : WB
Reactivity : Human
Muralimanoharan S et al. J Mol Cell Cardiol 2017; 108:181-193 Sexual dimorphism in the fetal cardiac response to maternal nutrient restriction.
Application : WB
Reactivity : Baboon
Zhang S et al. Reprod Fertil Dev 2017; (Epub) Prostaglandin F2--PTGFR signalling activation, growth factor expression and cell proliferation in bovine endometrial explants.
Application : WB
Reactivity : Bovine
Yeh CC et al. Mol Cell Proteomics 2015; 14 (6):1527-45 integrated stable isotope labeling by amino acids in cell culture (silac) and isobaric tags for relative and absolute quantitation (itraq) quantitative proteomic analysis identifies galectin-1 as a potential biomarker for predicting * resistance in liver cancer.
Application : WB
Reactivity : Human
Qi XJ et al. Int J Clin Exp Pathol 2015; 8 (10):12140-50 Fasudil, an inhibitor of Rho-associated coiled-coil kinase, attenuates hyperoxia-induced pulmonary fibrosis in neonatal rats.
Application : WB
Reactivity : Rat
Xiu-Jie Qi et al. Int J Clin Exp Pathol. 2015; 8 (10):12140-12150 Fasudil, an inhibitor of Rho-associated coiled-coil kinase, attenuates hyperoxia-induced pulmonary fibrosis in neonatal rats.
Application : IHC-P
Reactivity : Rat
Davide P et al. Cell Mol Gastroenterol Hepatol 2015; 1 (6):646-663 Lipid-Induced Hepatocyte-Derived Extracellular Vesicles Regulate Hepatic Stellate Cells via MicroRNA Targeting Peroxisome Proliferator-Activated Receptor γ.
Application : WB
Reactivity : Human
Taniguchi K et al. Nature 2015; 519 (7541):57-62 A gp130-Src-YAP module links inflammation to epithelial regeneration.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169