GeneTex
United States (US)

CTGF antibody

Orthogonal Overexpression
GTX124233_40653_20180831_WB_Fraction_18121410_534.jpg
GTX124233_40653_20160427_IHC-P_M_18121410_153.jpg
GTX124233_40653_WB_18121410_262.jpg
GTX124233_40653_20180817_WB_B_18121410_279.jpg
GTX124233_40653_WB_R_Bladder_18121410_706.jpg

Cat No. GTX124233

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HUVEC , Rat bladder , CTGF-transfected 293T

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CTGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Connective Tissue Growth Factor,Ccn2,Hcs24,Igfbp8,Nov2,Ctgf

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124233_40653_20180831_WB_Fraction_18121410_534.jpg

GTX124233 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX124233_40653_20160427_IHC-P_M_18121410_153.jpg

GTX124233 IHC-P Image

CTGF antibody detects secreted CTGF protein in mouse placenta by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
CTGF antibody (GTX124233) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124233_40653_WB_18121410_262.jpg

GTX124233 WB Image

Sample (30 ug of whole cell lysate)
A: HUVEC
10% SDS PAGE
GTX124233 diluted at 1:1000

GTX124233_40653_20180817_WB_B_18121410_279.jpg

GTX124233 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124233_40653_WB_R_Bladder_18121410_706.jpg

GTX124233 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX124233 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169