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CTH antibody

KO/KD
Anti-CTH antibody used in Western Blot (WB). GTX113409
Anti-CTH antibody used in Western Blot (WB). GTX113409
Anti-CTH antibody used in IHC (Paraffin sections) (IHC-P). GTX113409
Anti-CTH antibody used in IHC (Paraffin sections) (IHC-P). GTX113409
Anti-CTH antibody used in Western Blot (WB). GTX113409
Anti-CTH antibody used in Western Blot (WB). GTX113409

Cat. No. GTX113409

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CTH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse liver , rat liver , 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystathionine gamma-lyase

Cellular Localization

Cytoplasm

Background

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CTH antibody used in Western Blot (WB). GTX113409

GTX113409 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTH antibody (GTX113409) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTH antibody used in Western Blot (WB). GTX113409

GTX113409 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTH antibody (GTX113409) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTH antibody used in IHC (Paraffin sections) (IHC-P). GTX113409

GTX113409 IHC-P Image

CTH antibody detects CTH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CTH stained by CTH antibody (GTX113409) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CTH antibody used in IHC (Paraffin sections) (IHC-P). GTX113409

GTX113409 IHC-P Image

CTH antibody detects CTH protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
CTH antibody (GTX113409) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CTH antibody used in Western Blot (WB). GTX113409

GTX113409 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CTH antibody (GTX113409) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTH antibody used in Western Blot (WB). GTX113409

GTX113409 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTH antibody (GTX113409) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zheng J et al. Cell Death Dis 2021; 12 (7) : 698 Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines.
Application : WB
Reactivity : Human
Li K et al. Food Funct 2018; (Epub) The protective effect of polyunsaturated fatty acids intake during pregnancy against embryotoxicity of sodium valproate in mice.
Application : WB
Reactivity : Mouse
Uren PJ et al. RNA Biol 2016; 13 (4) : 400-11 High-throughput analyses of hnRNP H1 dissects its multi-functional aspect.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169