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CTLA4 antibody [BNI3] (PE)

Anti-CTLA4 antibody [BNI3] (PE) used in Flow cytometry (FACS). GTX00492-08

Cat No. GTX00492-08

Host

Mouse

Clonality

Monoclonal

Clone Name

BNI3

Isotype

IgG2a

Application

FACS

Reactivity

Human, Baboon, Cynomolgus monkey, Rhesus Monkey
Package
100 test ($329)
PRODUCT

Summary

The BNI3 antibody is specific for human CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml

Antigen Species

Human

Immunogen

Human CD152-IgG heavy chain fusion protein

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytotoxic T-lymphocyte associated protein 4 , ALPS5 , CD , CD152 , CELIAC3 , CTLA-4 , GRD4 , GSE , IDDM12

Cellular Localization

Cell membrane

Background

This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CTLA4 antibody [BNI3] (PE) used in Flow cytometry (FACS). GTX00492-08

GTX00492-08 FACS Image

FACS analysis of human PBMC stimulated overnight with PMA and Ionomycin stained APC anti-human CD4 antibody, followed by intracellular staining with GTX00492-08 CTLA4 antibody [BNI3] (PE).
Right panel : Co-stained with APC anti-human CD4 antibody and GTX00492-08
Left panel : Co-stained with APC anti-human CD4 antibody and PE mouse IgG2a isotype control
Antibody amount : 0.5 μg

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329