Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody (GTX134527) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CTLA4 antibody detects CTLA4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
CTLA4 stained by CTLA4 antibody (GTX134527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody (GTX134527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.