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CTP synthase antibody [N1C1]

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265
Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

Cat No. GTX105265

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTP synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036687

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CTP synthase 1 , CTPS , GATD5 , IMD24

Background

The catalytic conversion of UTP to CTP is accomplished by the enzyme cytidine-5-prime-triphosphate synthetase. The enzyme is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis. The region to which the CTPS gene has been mapped is the location of breakpoints involved in several tumor types [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

GTX105265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CTP synthase antibody [N1C1] (GTX105265) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

GTX105265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CTP synthase antibody [N1C1] (GTX105265) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

CTP synthase antibody [N1C1] Cat No. GTX105265

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Sci in 2015.PMID: 26303200
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Cell Sci in 2015.PMID: 26303200
Date : Anonymous submitted on 19-Jul-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : HEK293T cells transfected with mouse CTPs-EGFP
Blocking : 1% BSA, 25°C, 0.5Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.2% Triton-X
Primary Antibody : 1:200, 25°C, 1Hr
Figure Note : Green: EGFP, Magenta: CTPs (detected with anti-rabbit-IgG Alexa647), Blue: DAPI
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biol Open in 2014.PMID: 25326513
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Biol Open in 2014.PMID: 25326513
SDS
Glycerol.pdf
Thimerosal.pdf