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CUGBP1 antibody [5B8]

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692
Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692

Cat No. GTX84692

Host

Mouse

Clonality

Monoclonal

Clone Name

5B8

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Monkey
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CUGBP1 (NP_006551) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CUGBP Elav-like family member 1 , BRUNOL2 , CUG-BP , CUGBP , CUGBP1 , EDEN-BP , NAB50 , NAPOR , hNab50

Cellular Localization

Nucleus,Cytoplasm

Background

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.

Database

Research Area

DATA IMAGES

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of kidney tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84692

GTX84692 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CUGBP1 plasmid using GTX84692 CUGBP1 antibody [5B8].

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692

GTX84692 WB Image

WB analysis of various cell lines using GTX84692 CUGBP1 antibody [5B8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692

GTX84692 WB Image

WB analysis of HEK293T cells transfected with CUGBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84692 CUGBP1 antibody [5B8].
Loading : 5 ug per lane

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of liver tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of prostate tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX84692

GTX84692 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84692 CUGBP1 antibody [5B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CUGBP1 antibody [5B8] used in Western Blot (WB). GTX84692

GTX84692 WB Image

WB analysis of various samples using GTX84692 CUGBP1 antibody [5B8].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf