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CUGBP2 antibody [N1N3]

Anti-CUGBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115463
Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463
Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463
Anti-CUGBP2 antibody [N1N3] used in Western Blot (WB). GTX115463
Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463

Cat. No. GTX115463

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CUGBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CUGBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CUGBP Elav-like family member 2 , BRUNOL3 , CELF-2 , CUG-BP2 , CUGBP2 , ETR-3 , ETR3 , NAPOR

Cellular Localization

Nucleus , Cytoplasm

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CUGBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115463

GTX115463 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using CUGBP2(GTX115463) antibody at 1:500 dilution.

Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463

GTX115463 IHC-P Image

CUGBP2 antibody [N1N3] detects CUGBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CUGBP2 antibody [N1N3] (GTX115463) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463

GTX115463 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using CUGBP2(GTX115463) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CUGBP2 antibody [N1N3] used in Western Blot (WB). GTX115463

GTX115463 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX115463 diluted at 1:3000

Anti-CUGBP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115463

GTX115463 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CUGBP2(GTX115463) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169