GeneTex
United States (US)

CUGBP2 antibody [N1N3]

GTX115463_40401_IHC_R_18121410_754.jpg
GTX115463_40401_WB_18121410_766.jpg
GTX115463_40401_IHC_18121410_614.jpg
GTX115463_40401_IFA_18121410_667.jpg
GTX115463_40401_20150724_IHC-P_M_18121410_991.jpg

Cat No. GTX115463

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , MCF-7

Predict Reactivity

Zebrafish, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CUGBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cugbp Elav-Like Family Member 2,Brunol3,Celf-2,Cug-Bp2,Cugbp2,Etr-3,Etr3,Napor,Celf2

Cellular Localization

Nucleus , Cytoplasm

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115463_40401_IHC_R_18121410_754.jpg

GTX115463 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using CUGBP2(GTX115463) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115463_40401_WB_18121410_766.jpg

GTX115463 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX115463 diluted at 1:3000

GTX115463_40401_IHC_18121410_614.jpg

GTX115463 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CUGBP2(GTX115463) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115463_40401_IFA_18121410_667.jpg

GTX115463 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using CUGBP2(GTX115463) antibody at 1:500 dilution.

GTX115463_40401_20150724_IHC-P_M_18121410_991.jpg

GTX115463 IHC-P Image

CUGBP2 antibody [N1N3] detects CUGBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CUGBP2 antibody [N1N3] (GTX115463) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169