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CUTC antibody [N1C2]

Anti-CUTC antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119839
Anti-CUTC antibody [N1C2] used in Western Blot (WB). GTX119839
Anti-CUTC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119839

Cat. No. GTX119839

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CUTC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CUTC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724485

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cutC copper transporter , CGI-32

Background

Members of the CUT family of copper transporters are associated with copper homeostasis and are involved in the uptake, storage, delivery, and efflux of copper (Gupta et al., 1995 [PubMed 7635807]; Li et al., 2005 [PubMed 16182249]).[supplied by OMIM]

Database

DATA IMAGES

Anti-CUTC antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119839

GTX119839 ICC/IF Image

CUTC antibody [N1C2] detects CUTC protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CUTC protein stained by CUTC antibody [N1C2] (GTX119839) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CUTC antibody [N1C2] used in Western Blot (WB). GTX119839

GTX119839 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX119839 diluted at 1:2000

Anti-CUTC antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119839

GTX119839 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CUTC(GTX119839) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169