APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Positive Control
Raji , K562
Predict Reactivity
Mouse, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CUTL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11164725
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cut like homeobox 1 , CASP , CDP , CDP/Cut , CDP1 , COY1 , CUTL1 , CUX , Clox , Cux/CDP , GDDI , GOLIM6 , Nbla10317 , p100 , p110 , p200 , p75
Cellular Localization
Nucleus,Golgi apparatus membrane
Background
The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX114351 IP Image
CUTL1 antibody immunoprecipitates CUTL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CUTL1 protein by 2 μg of CUTL1 antibody (GTX114351) 7.5% SDS-PAGE The immunoprecipitated CUTL1 protein was detected by CUTL1 antibody (GTX114351) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX114351 ICC/IF Image
CUTL1 antibody detects CUTL1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CUTL1 stained by CUTL1 antibody (GTX114351) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX114351 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
B: K562
5% SDS PAGE
GTX114351 diluted at 1:500
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GTX114351 ICC/IF Image
CUTL1 antibody detects CUTL1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CUTL1 protein stained by CUTL1 antibody (GTX114351) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
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REVIEW
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