100 μl ($319),
25 μl ($169)
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
GTX132714 WB Image
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CX3CR1 antibody (GTX132714) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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