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CX3CR1 antibody

KO/KD
Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260
Anti-CX3CR1 antibody used in Flow cytometry (FACS). GTX31260
Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260
Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260
Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

Cat. No. GTX31260

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CX3CR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2 μg/mL
FACS 0.1 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921) , Mouse Kidney Tissue Lysate(GTX27936) , Rat Spleen Tissue Lysate(GTX27945)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CX3CR1 antibody was raised against a 20 amino acid peptide near the amino terminus of human CX3CR1.The immunogen is located within the first 50 amino acids of CX3CR1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X3-C motif chemokine receptor 1 , CCRL1 , CMKBRL1 , CMKDR1 , GPR13 , GPRV28 , V28

Cellular Localization

Cell membrane

Background

Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260

GTX31260 WB Image

WB analysis of rat spleen tissue lysate using GTX31260 CX3CR1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-CX3CR1 antibody used in Flow cytometry (FACS). GTX31260

GTX31260 FACS Image

FACS analysis of THP-1 cells using GTX31260 CX3CR1 antibody.
Working concentration : 0.1 μg/ml
Green: Isotype control
Red : CX3CR1 antibody

Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260

GTX31260 WB Image

KO/KD

WB analysis of 293 cell or CX3CR1 KD cell lysates using GTX31260 CX3CR1 antibody.
Lane 1 : 293 cells transfected with control siRNAs
Lane 2 : 293 cells transfected with CX3CR1 siRNAs
Dilution : 0.5μg/mL
Loading : 15μg

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 20 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of human heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 2 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 2 μg/ml

Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260

GTX31260 WB Image

WB analysis of mouse kidney tissue lysate using GTX31260 CX3CR1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of human heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 10 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 2 μg/ml

Anti-CX3CR1 antibody used in Western Blot (WB). GTX31260

GTX31260 WB Image

WB analysis of human spleen lysate in the absence (lane 1) or presence of blocking peptide (lane 2) using GTX31260 CX3CR1 antibody.
Working concentration : 1 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31260

GTX31260 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31260 CX3CR1 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Rocha-Neto LM et al. Neuroscience 2019; 416 : 229-238 Early Phase of Type 1 Diabetes Decreases the Responsiveness of C-Fiber Nociceptors in the Temporomandibular Joint of Rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399