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CX3CR1 antibody

Anti-CX3CR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31648
Anti-CX3CR1 antibody used in Western Blot (WB). GTX31648
Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31648

Cat. No. GTX31648

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all CX3CR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL
ICC/IF Assay dependent
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

THP-1 Cell Slide(GTX25540) , THP-1 Cell Lysate(GTX27913) , 3T3 Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1.The immunogen is located within amino acids 160 - 210 of CX3CR1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X3-C motif chemokine receptor 1 , CCRL1 , CMKBRL1 , CMKDR1 , GPR13 , GPRV28 , V28

Cellular Localization

Cell membrane

Background

Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-CX3CR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31648

GTX31648 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX31648 CX3CR1 antibody.
Working concentration : 20 μg/ml

Anti-CX3CR1 antibody used in Western Blot (WB). GTX31648

GTX31648 WB Image

WB analysis of THP-1 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31648 CX3CR1 antibody.
Working concentration : 1 μg/ml

Anti-CX3CR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31648

GTX31648 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31648 CX3CR1 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399