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CXCL1 / GRO alpha antibody

Anti-CXCL1 / GRO alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31184
Anti-CXCL1 / GRO alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31184

Cat No. GTX31184

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA, Neutralizing/Inhibition

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
For WB: Use at a concentration of 0.1 - 0.2 μg/ml.
For IHC-P: Use at a concentration of 0.125 –0.150 μg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml.
For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Human GRO/MGSA (100 ng/ml), a concentration of 1.5-3.0 μg/ml of this antibody is required.
Optimal dilution should be determined by the end user.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human GRO-α/MGSA

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C Motif Chemokine Ligand 1 , Fsp , Gro1 , Groa , Mgsa , Mgsa-A , Nap-3 , Scyb1 , Cxcl1

Background

This gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signal through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-CXCL1 / GRO alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31184

GTX31184 IHC-P Image

IHC-P analysis of human colon/rectum adenocarcinoma using GRO alpha antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

Anti-CXCL1 / GRO alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31184

GTX31184 IHC-P Image

IHC-P analysis of human colon/rectum adenocarcinoma using GRO alpha antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 319