GeneTex
United States (US)

CXCL10 / IP10 antibody

GTX31176_20150924_ELISA_18121410_138.jpg
GTX31176_IHC-P_1_18121410_647.jpg
GTX31176_IHC-P_18121410_335.jpg

Cat No. GTX31176

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 1.0 μg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Human IP-10 (100.0 ng/ml), a concentration of 5.0 – 8.0 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

11 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Human IP-10 (CXCL10)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 10,C7,Ifi10,Inp10,Ip-10,Scyb10,Crg-2,Gip-10,Mob-1,Cxcl10

Background

This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX31176_20150924_ELISA_18121410_138.jpg

GTX31176 ELISA Image

Sandwich To detect hIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody- which is biotinylated (GTX31176) allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.

GTX31176_IHC-P_1_18121410_647.jpg

GTX31176 IHC-P Image

IHC-P analysis of human carcinoid tissue using IP10 antibody at a concentration of 1.0 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

GTX31176_IHC-P_18121410_335.jpg

GTX31176 IHC-P Image

IHC-P analysis of human carcinoid tissue using IP10 antibody at a concentration of 1.0 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

REFERENCE
Package List Price ($)
$ 319