GeneTex
United States (US)

CXCL11 antibody

GTX31187_20150924_ELISA_18121410_355.jpg
GTX31187_IHC-P_18121410_993.jpg
GTX31187_IHC-P_1_18121410_448.jpg

Cat No. GTX31187

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.125μg/mL- 0.25μg/mL. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 7.0-8.0 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

10 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Human I-TAC (CXCL11)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 11,H174,I-Tac,Ip-9,Ip9,Scyb11,Scyb9B,B-R1,Cxcl11

Background

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX31187_20150924_ELISA_18121410_355.jpg

GTX31187 ELISA Image

Sandwich To detect hI-TAC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody- which is biotinylated (GTX31187) allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.

GTX31187_IHC-P_18121410_993.jpg

GTX31187 IHC-P Image

IHC-P analysis of human renal tumor with parenchyma tissue using CXCL11 antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval.

GTX31187_IHC-P_1_18121410_448.jpg

GTX31187 IHC-P Image

IHC-P analysis of human renal tumor with parenchyma tissue using CXCL11 antibody. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval.

Package List Price ($)
$ 319