GeneTex
United States (US)

CXCL14 antibody [N3C3]

GTX108431_IHC2_19011507_667.jpg
GTX108431_IHC1_19011507_296.jpg
GTX108431_39848_WB_M_brain_19011507_944.jpg
GTX108431_39848_20141017_WB_19011507_577.jpg

Cat No. GTX108431

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 14,Bmac,Brak,Kec,Ks1,Mip-2G,Mip2G,Njac,Scyb14,Cxcl14

Cellular Localization

Secreted

Background

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108431_IHC2_19011507_667.jpg

GTX108431 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma , using CXCL14(GTX108431) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108431_IHC1_19011507_296.jpg

GTX108431 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma , using CXCL14(GTX108431) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108431_39848_WB_M_brain_19011507_944.jpg

GTX108431 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX108431 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108431_39848_20141017_WB_19011507_577.jpg

GTX108431 WB Image

BRAK antibody detects BRAK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with BRAK antibody (GTX108431) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf