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CXCL16 antibody [GT516]

Recombinant antibody Orthogonal Overexpression
Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502
Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502
Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502
Anti-CXCL16 antibody [GT516] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632502
Anti-CXCL16 antibody [GT516] used in IHC (Paraffin sections) (IHC-P). GTX632502

Cat. No. GTX632502

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT516

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CXCL16 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

CXCL16-transfected 293T

Product Note

This antibody was raised against human CXCL16 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CXCL16. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 16 , CXCLG16 , SR-PSOX , SRPSOX

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Secreted , Cell membrane , Secreted

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Database

Research Area

DATA IMAGES

Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

GTX632502 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

GTX632502 WB Image

Orthogonal

HeLa whole cell extract and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-CXCL16 antibody [GT516] used in Western Blot (WB). GTX632502

GTX632502 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CXCL16 antibody [GT516] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632502

GTX632502 ICC/IF Image

CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCL16 stained by CXCL16 antibody [GT516] (GTX632502) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CXCL16 antibody [GT516] used in IHC (Paraffin sections) (IHC-P). GTX632502

GTX632502 IHC-P Image

CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
CXCL16 antibody [GT516] (GTX632502) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Choi YW et al. Adv Sci (Weinh) 2021; 8 (4) : 2002497 Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.
Application : IHC-P
Reactivity : Mouse
Shibata Y et al. Thorac Cancer 2020; 11 (5) : 1258-1264 The clinical significance of CXCL16 in the treatment of advanced non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169