CXCL16 antibody [GT5212]

Cat. No. GTX632390

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5212
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CXCL16 products

Datasheet
Document

View product citations for antibody GTX632390 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

human CXCL16-transfected 293T

Product Note

This antibody was raised against human CXCL16 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CXCL16. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888312

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 16 , CXCLG16 , SR-PSOX , SRPSOX

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Secreted , Cell membrane , Secreted

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Database

Gene ID: 58191 CXCL16
UniProt: Q9H2A7 CXCL16

Research Area

DATA IMAGES

GTX632390 IHC-P Image

CXCL16 antibody [GT5212] detects secreted CXCL16 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric cancer.
CXCL16 stained by CXCL16 antibody [GT5212] (GTX632390) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632390 WB Image

CXCL16 antibody [GT5212] detects CXCL16 protein by western blot analysis. Non-transfected (-) and CXCL16-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT5212] (GTX632390) at a dilution of 1:1000.

GTX632390 ICC/IF Image

CXCL16 antibody [GT5212] detects CXCL16 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCL16 stained by CXCL16 antibody [GT5212] (GTX632390) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations