GeneTex
United States (US)

CXCL8 / IL8 antibody [3IL8-H10]

Orthogonal
GTX15763_298_ICC-IF_19011519_459.jpg
GTX15763_65_ELISA_19011519_290.jpg

Cat No. GTX15763

Host Mouse
Clonality Monoclonal
Clone Name 3IL8-H10
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 2 μg/ml
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-8

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 8,Gcp-1,Gcp1,Il8,Lect,Luct,Lynap,Mdncf,Monap,Naf,Nap-1,Nap1,Cxcl8

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
GTX15763_298_ICC-IF_19011519_459.jpg

GTX15763 ICC/IF Image

Orthogonal

ICC/IF analysis of U-87 MG cells treated 1 uM thapsigargin for 24 hours using GTX15763 CXCL8 / IL8 antibody [3IL8-H10]. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute

GTX15763_65_ELISA_19011519_290.jpg

GTX15763 ELISA Image

Sandwich ELISA analysis of serial diluted human IL-8 recombinant protein(50ul per well) using GTX15763 CXCL8 / IL8 antibody [3IL8-H10] (capture antibody) at 3 µg/mL and biotinylated mouse anti-human IL-8 monoclonal antibody (detection antibody) at 0.25 µg/mL.

SDS
PBS.pdf