GeneTex
United States (US)

CXCL8 / IL8 antibody [6217]

Cat No. GTX10768

Host Mouse
Clonality Monoclonal
Clone Name 6217
Isotype IgG1
Application WB, ICC/IF, FACS, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ICC/IF 25 μg/ml
FACS 25 μg/ml (10 μl/1-5 X 10⁵ cells)
ELISA 0.5 μg/ml
Neut 0.08-0.4 μg/ml
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

purified, E. coli-derived, recombinant human IL-8.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 8,Gcp-1,Gcp1,Il8,Lect,Luct,Lynap,Mdncf,Monap,Naf,Nap-1,Nap1,Cxcl8

Background

IL8, formerly called monocyte derived neutrophil chemotactic factor, belongs to the chemokine alpha family. Human endothelial IL8 is an 8.9 kDa protein containing 77 amino acid residues. IL8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils and promotes degranulation.

Database

Research Area