APPLICATION
Application Note
For ELISA: Use at an assay dependent dilution. Can be used as a detection antibody in an ELISA pair with GTX18649 (capture). For Neut: Use at a concentration of 5 μg/ml with 10 ng/ml IL8 for 98.4% inhibition of chemotactic activity. For WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
This antibody recognises both recombinant and native IL8. It does not cross react with Monocyte Chemotactic Activating Factor (MCAF)or Regulated on Activation, Normal T-cell Expressed, and Secreted (RANTES).
PROPERTIES
Form
Liquid
Buffer
PBS pH7.0
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant (Human).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-X-C motif chemokine ligand 8 , GCP-1 , GCP1 , IL8 , LECT , LUCT , LYNAP , MDNCF , MONAP , NAF , NAP-1 , NAP1
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656](/templates/default/js/lightbox/images/loading.gif "Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656")
|
GTX18656 WB Image
Western blot analysis of Human IL-8 using clone I8-60. Concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of human IL-8.
|
REFERENCE
There are currently no references for CXCL8 / IL8 antibody [I8-60] (GTX18656). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CXCL8 / IL8 antibody [I8-60] (GTX18656). Be the first to share your experience with this product.
![Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656](/upload/website/prouct_img/small/GTX18656/IL8-antibody-I8-60__GTX18656-1_w_23060620_295.webp "Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656")
![Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656](/upload/website/prouct_img/normal/GTX18656/IL8-antibody-I8-60__GTX18656-1_w_23060620_295.webp "Anti-CXCL8 / IL8 antibody [I8-60] used in Western Blot (WB). GTX18656")