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CXCR1 antibody [C2C3], C-term

Anti-CXCR1 antibody [C2C3], C-term used in Western Blot (WB). GTX100389
Anti-CXCR1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100389
Anti-CXCR1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100389
Anti-CXCR1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100389
Anti-CXCR1 antibody [C2C3], C-term used in Western Blot (WB). GTX100389

Cat No. GTX100389

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2 , mouse leukocyte treated with CFA

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine receptor 1 , C-C , C-C-CKR-1 , CD128 , CD181 , CDw128a , CKR-1 , CMKAR1 , IL8R1 , IL8RA , IL8RBA

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR1 antibody [C2C3], C-term used in Western Blot (WB). GTX100389

GTX100389 WB Image

Untreated (–) and treated (+) mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR1 antibody [C2C3], C-term (GTX100389) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (CFA: Complete Freunds adjuvant)

Anti-CXCR1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100389

GTX100389 IHC-P Image

CXCR1 antibody [C2C3], C-term detects IL8RA protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
CXCR1 antibody [C2C3], C-term (GTX100389) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCR1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100389

GTX100389 IHC-P Image

CXCR1 antibody [C2C3], C-term detects CXCR1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: CXCR1 antibody [C2C3], C-term (GTX100389) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CXCR1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100389

GTX100389 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using CXCR1(GTX100389) antibody at 1:500 dilution.

Anti-CXCR1 antibody [C2C3], C-term used in Western Blot (WB). GTX100389

GTX100389 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX100389 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

CXCR1 antibody [C2C3], C-term Cat No. GTX100389

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Feb-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : CT26 cells
Blocking : 2% FBS in PBS, 25°C, 0.15Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169