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CXCR2 antibody [GT547]

Anti-CXCR2 antibody [GT547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631667
Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667

Cat No. GTX631667

Host

Mouse

Clonality

Monoclonal

Clone Name

GT547

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

3xFlag-human CXCR2-transfected 293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CXCR2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888251

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine receptor 2 , CD182 , CDw128b , CMKAR2 , IL8R2 , IL8RA , IL8RB

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CXCR2 antibody [GT547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631667

GTX631667 ICC/IF Image

CXCR2 antibody [GT547] detects CXCR2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR2 stained by CXCR2 antibody [GT547] (GTX631667) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-CXCR2 antibody [GT547] used in Western Blot (WB). GTX631667

GTX631667 WB Image

CXCR2 antibody detects CXCR2 protein by western blot analysis. Non-transfected (-) and CXCR2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody (GTX631667) diluted by 1:5000.

REFERENCE

There are currently no references for CXCR2 antibody [GT547] (GTX631667). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CXCR2 antibody [GT547] (GTX631667). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169