APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
3xFlag-human CXCR2-transfected 293T
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CXCR2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888251
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-X-C motif chemokine receptor 2 , CD182 , CDw128b , CMKAR2 , IL8R2 , IL8RA , IL8RB
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX631667 ICC/IF Image
CXCR2 antibody [GT547] detects CXCR2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CXCR2 stained by CXCR2 antibody [GT547] (GTX631667) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX631667 WB Image
Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [GT547] (GTX631667) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX631667 WB Image
CXCR2 antibody detects CXCR2 protein by western blot analysis. Non-transfected (-) and CXCR2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody (GTX631667) diluted by 1:5000.
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REFERENCE
There are currently no references for CXCR2 antibody [GT547] (GTX631667). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CXCR2 antibody [GT547] (GTX631667). Be the first to share your experience with this product.