CXCR2 antibody [HL2604]

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:200 and competitor's antibody (Highly Cited Competitor) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Unboiled THP-1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000 and competitor's antibody (Clone 48311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

CXCR2 antibody [HL2604] (GTX639056) detects CXCR2 protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CXCR2 antibody [HL2604] detects CXCR2 protein by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) unboiled THP-1 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CXCR2 antibody [HL2604] (GTX639056) specifically detects CXCR2 protein on a VirD^TM-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:1000 dilution.

Yellow: CXCR2
Blue: CXCR1, CXCR3, CXCR4, CXCR5, or CXCR6
White: Positive control

CXCR2 antibody [HL2604] detects CXCR2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
CXCR2 stained by CXCR2 antibody [HL2604] (GTX639056) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [HL2604] (GTX639056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.